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biotinylated anti mouse ifn γ detection antibody  (Mabtech Inc)

 
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    Mabtech Inc biotinylated anti mouse ifn γ detection antibody
    Biotinylated Anti Mouse Ifn γ Detection Antibody, supplied by Mabtech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+mouse+ifn+%CE%B3+detection+antibody/anti+biotinylated+detection+ifn+mouse+%CE%B3/bio_rxiv__64898__2026__05__13__723724-228-6-12
    Average 86 stars, based on 1 article reviews
    biotinylated anti mouse ifn γ detection antibody - by Bioz Stars, 2026-10
    86/100 stars

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    Incubation:

    Article Title: Characterization of a factor VIII/immunoglobulin heavy chain μ double-knockout mouse model of hemophilia A for long-term exposure to factor VIII proteins
    Article Snippet: .. After incubation at 37 °C and 5% CO 2 for either 15 or 39 hours, cells were washed 5 times with PBS and incubated for 2 hours at RT with a biotinylated anti-mouse IFN-γ detection antibody (R4-6A2-biotin [part of the ELISPOT kit from Mabtech], 1 μg/mL in PBS + 0.5% HI FBS). .. After 5 additional PBS washes, streptavidin-ALP secondary antibody (part of the ELISPOT kit from Mabtech) was added at a 1:1000 dilution (PBS + 0.5% HI FBS) and incubated for 1 hour at RT.

    Article Title: Generation of an autophagy-targeting influenza A virus as live attenuated vaccine.
    Article Snippet: Interferon (IFN)-γ enzyme-linked immunospot (ELISpot) assay Viral antigen-specific T cellular responses were detected by an IFN-γ ELISpot kit (Mabtech, Cat# 3321-4AST-2) according to the manufacturer’s instructions. .. After 24 h of incubation at 37 °C with 5% CO2, plates were washed and incubated with biotinylated anti-mouse IFN-γ detection antibody (MABTECH, Cat# 3321-6; 1:1000 dilution), followed by streptavidinALP (MABTECH, Cat# 3310-10; 1:1000 dilution) and substrate BCIP/NBT-Plus for ALP (MABTECH, Cat# 3650-10). .. Cells were plated at 5 × 105 cells per well in triplicate and restimulated with influenza virus–derived peptides at a final concentration of 10μg/mL in RPMI 1640 (Gibco, Cat# C11875500BT).

    Article Title: Characterization of a FVIII/Ighm Double Knockout mouse model of hemophilia A for long-term exposure to factor VIII proteins
    Article Snippet: .. After incubation at 37 ◦C and 5% CO2 for either 15 or 39 hours, cells were washed 5 times with PBS and incubated for 2 hours at RT with a biotinylated anti-mouse IFN-γ detection antibody (R46A2-biotin [part of the ELISPOT kit from Mabtech], 1 μg/mL in PBS + 0.5% HI FBS). .. After 5 additional PBS washes, streptavidinALP secondary antibody (part of the ELISPOT kit from Mabtech) was added at a 1:1000 dilution (PBS + 0.5% HI FBS) and incubated for 1 hour at RT.

    Article Title: Acquired resistance to immune checkpoint inhibitors is associated with hypoxia and ECM remodeling in colorectal cancer
    Article Snippet: A positive control included stimulation with concanavalin A (5 μg/mL; C5275, Sigma Aldrich), while unstimulated cells were a negative control. .. After overnight incubation at 37°C, plates were developed using a biotinylated anti-mouse IFN-γ detection antibody (3321-6-250, MabTech), streptavidin-ALP (3310-10, MabTech), and BCIP/NBT substrate (3650-10, MabTech). .. Spots were quantified using a CTL ImmunoSpot S6 analyzer with ImmunoSpot software (v5.1, CTL Analyzers).

    Enzyme-linked Immunospot:

    Article Title: Characterization of a factor VIII/immunoglobulin heavy chain μ double-knockout mouse model of hemophilia A for long-term exposure to factor VIII proteins
    Article Snippet: .. After incubation at 37 °C and 5% CO 2 for either 15 or 39 hours, cells were washed 5 times with PBS and incubated for 2 hours at RT with a biotinylated anti-mouse IFN-γ detection antibody (R4-6A2-biotin [part of the ELISPOT kit from Mabtech], 1 μg/mL in PBS + 0.5% HI FBS). .. After 5 additional PBS washes, streptavidin-ALP secondary antibody (part of the ELISPOT kit from Mabtech) was added at a 1:1000 dilution (PBS + 0.5% HI FBS) and incubated for 1 hour at RT.

    Article Title: Characterization of a FVIII/Ighm Double Knockout mouse model of hemophilia A for long-term exposure to factor VIII proteins
    Article Snippet: .. After incubation at 37 ◦C and 5% CO2 for either 15 or 39 hours, cells were washed 5 times with PBS and incubated for 2 hours at RT with a biotinylated anti-mouse IFN-γ detection antibody (R46A2-biotin [part of the ELISPOT kit from Mabtech], 1 μg/mL in PBS + 0.5% HI FBS). .. After 5 additional PBS washes, streptavidinALP secondary antibody (part of the ELISPOT kit from Mabtech) was added at a 1:1000 dilution (PBS + 0.5% HI FBS) and incubated for 1 hour at RT.



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    biotinylated anti mouse ifn γ detection antibody  (Mabtech Inc)
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    biotinylated anti-mouse ifn γ detection antibody  (Becton Dickinson)
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    Splenocyte composition, insulitis, and autoreactive CD8 T cell specificities in NOD. β 2 m KO .HHD.Ins2 het mice. (a) and (b) Splenocytes from 8-week-old female NOD. β 2 m KO .HHD (filled circles) and NOD. β 2 m KO .HHD.Ins2 het mice (open circles) were analyzed by flow cytometry. Each symbol represents an individual mouse. (c) Female NOD. β 2 m KO .HHD (filled circles) and NOD. β 2 m KO .HHD.Ins2 het mice (open circles) were sacrificed at 4 and 8 weeks of age and insulitis indices were determined as described in Materials and Methods and plotted. Each symbol represents an individual mouse. ∗ p = 0.0037 (Mann-Whitney U ). Representative islets from a single NOD. β 2 m KO .HHD.Ins2 het mouse are also shown. In these images, beta cells appear dark purple and are denoted by red arrows, while the more lightly stained infiltrating immune cells are marked by black arrows. The number on each image indicates the insulitis score of the islet shown. (d) Islet-infiltrating cells from 8-week-old female NOD. β 2 m KO .HHD.Ins2 het mice were tested for reactivity to the indicated HLA-A ∗ 02:01-restricted insulin and IGRP epitopes by <t>IFN-</t> γ ELISPOT. Stimulation index was calculated by dividing the number of spots detected for a given peptide by the number of spots detected with an irrelevant HIV-derived HLA-A ∗ 02:01-binding peptide. A stimulation index greater than 2 was considered a positive response.
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    Image Search Results


    Splenocyte composition, insulitis, and autoreactive CD8 T cell specificities in NOD. β 2 m KO .HHD.Ins2 het mice. (a) and (b) Splenocytes from 8-week-old female NOD. β 2 m KO .HHD (filled circles) and NOD. β 2 m KO .HHD.Ins2 het mice (open circles) were analyzed by flow cytometry. Each symbol represents an individual mouse. (c) Female NOD. β 2 m KO .HHD (filled circles) and NOD. β 2 m KO .HHD.Ins2 het mice (open circles) were sacrificed at 4 and 8 weeks of age and insulitis indices were determined as described in Materials and Methods and plotted. Each symbol represents an individual mouse. ∗ p = 0.0037 (Mann-Whitney U ). Representative islets from a single NOD. β 2 m KO .HHD.Ins2 het mouse are also shown. In these images, beta cells appear dark purple and are denoted by red arrows, while the more lightly stained infiltrating immune cells are marked by black arrows. The number on each image indicates the insulitis score of the islet shown. (d) Islet-infiltrating cells from 8-week-old female NOD. β 2 m KO .HHD.Ins2 het mice were tested for reactivity to the indicated HLA-A ∗ 02:01-restricted insulin and IGRP epitopes by IFN- γ ELISPOT. Stimulation index was calculated by dividing the number of spots detected for a given peptide by the number of spots detected with an irrelevant HIV-derived HLA-A ∗ 02:01-binding peptide. A stimulation index greater than 2 was considered a positive response.

    Journal: Journal of Diabetes Research

    Article Title: An HLA-Transgenic Mouse Model of Type 1 Diabetes That Incorporates the Reduced but Not Abolished Thymic Insulin Expression Seen in Patients

    doi: 10.1155/2016/7959060

    Figure Lengend Snippet: Splenocyte composition, insulitis, and autoreactive CD8 T cell specificities in NOD. β 2 m KO .HHD.Ins2 het mice. (a) and (b) Splenocytes from 8-week-old female NOD. β 2 m KO .HHD (filled circles) and NOD. β 2 m KO .HHD.Ins2 het mice (open circles) were analyzed by flow cytometry. Each symbol represents an individual mouse. (c) Female NOD. β 2 m KO .HHD (filled circles) and NOD. β 2 m KO .HHD.Ins2 het mice (open circles) were sacrificed at 4 and 8 weeks of age and insulitis indices were determined as described in Materials and Methods and plotted. Each symbol represents an individual mouse. ∗ p = 0.0037 (Mann-Whitney U ). Representative islets from a single NOD. β 2 m KO .HHD.Ins2 het mouse are also shown. In these images, beta cells appear dark purple and are denoted by red arrows, while the more lightly stained infiltrating immune cells are marked by black arrows. The number on each image indicates the insulitis score of the islet shown. (d) Islet-infiltrating cells from 8-week-old female NOD. β 2 m KO .HHD.Ins2 het mice were tested for reactivity to the indicated HLA-A ∗ 02:01-restricted insulin and IGRP epitopes by IFN- γ ELISPOT. Stimulation index was calculated by dividing the number of spots detected for a given peptide by the number of spots detected with an irrelevant HIV-derived HLA-A ∗ 02:01-binding peptide. A stimulation index greater than 2 was considered a positive response.

    Article Snippet: Wells were then washed with 0.05% Tween 20/PBS and biotinylated anti-mouse IFN γ detection antibody (BD Biosciences) was added for 2 hours at 37°C.

    Techniques: Flow Cytometry, MANN-WHITNEY, Staining, Enzyme-linked Immunospot, Derivative Assay, Binding Assay